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doi: 10.1371/journal.ppat.1004188

Transmission electron microscope observation The cells in the logarithmic phase were cultured on a wall for one day and then exposed to HT, followed by rinsing the cells with PBS, adding electron microscope fixative (Servicebio, Wuhan, China) and fixing them for 6 min at 37 C

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Finally, the supernatant containing the metabolites was collected, dried, and reconstituted in an appropriate solvent for liquid chromatography-mass spectrometry (LC-MS) analysis