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With the sub-toxic dose of 0.1 mM, the low gradient of 40 is temporary, with cells rapidly starting to recover the Tpx1-driven gradient of 300, while with the toxic dose of 0.5 mM the gradient collapse is much more sustained and does not recover even after 50 min

Therefore, individuals who consume alcohol heavily should ensure adequate thiamine supplementation before and during ALA therapy

It will be interesting to test whether these mutants, or other mutants defective for dauer pheromone sensing and/or signaling exhibit an increase or decrease in longevity