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For the initial hit validation screen, primary hits were manually picked from the original compound library, while secondary screening assays were performed with compounds that were re-ordered directly from ChemBridge

5 Discussion and perspectives In recent years, in OA, a complex degenerative joint disease, increasing research indicates that ferroptosis not only exists in the pathological process but is also closely related to pathological features such as articular cartilage degeneration, synovial inflammation, and subchondral bone changes