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Preparation (before you inject) Step 1: Gather everything Reconstituted peptide vial (already mixed) Alcohol swabs (2-3) Insulin syringe (new, sterile) Sharps container nearby Band-aid (optional) Step 2: Wash your hands Soap and water, 20 seconds Dry thoroughly Don't skip this (infection prevention) Step 3: Choose injection site Abdomen recommended for first time Locate spot 2+ inches from navel Visually inspect (no cuts, bruises, or redness) Drawing up the peptide Step 4: Clean the vial Wipe top of peptide vial with alcohol swab Let air dry 10-15 seconds Don't blow on it or wipe with anything else Step 5: Draw air into syringe Remove syringe from packaging Pull plunger back to amount of peptide you're drawing Example: If drawing 0.3ml peptide, pull to 30 units This air will equalize pressure in vial Step 6: Insert needle into vial Remove cap from needle Insert needle through rubber stopper on vial Push air from syringe into vial (prevents vacuum) Step 7: Draw peptide into syringe Turn vial upside down (needle pointing up) Pull plunger down to draw liquid into syringe Draw slightly more than needed Flick syringe to move air bubbles to top Push plunger to expel air and get exact dose Step 8: Remove needle from vial Withdraw needle from vial Set vial aside (recap and refrigerate) Hold syringe pointing up (air at top) Performing the injection Step 9: Clean injection site Wipe injection site with alcohol swab Circular motion, starting from center Let air dry 10-15 seconds (dry alcohol doesn't sting) Step 10: Pinch skin Use non-dominant hand Pinch fold of skin at injection site Should easily create 1-2 inch fold Step 11: Insert needle Hold syringe like a pencil or dart Aim at 45-90 degree angle into pinched skin Quick, confident insertion (hesitating hurts more) Insert about 1/2 inch depth Step 12: Inject peptide Slowly push plunger down Take 2-3 seconds to inject Injecting too fast can cause burning sensation Step 13: Withdraw needle Release pinched skin Pull needle straight out Don't recap needle Step 14: Apply pressure Use clean cotton ball or finger Apply gentle pressure to injection site 5-10 seconds Apply band-aid if needed (usually not necessary) Step 15: Dispose of needle Drop syringe directly into sharps container Never recap used needles (stick injury risk) Don't leave needles lying around After injection What's normal: Tiny pinprick mark No bleeding or minimal drop No pain or slight tenderness Possible very small bump (goes away in minutes) What's not normal (call doctor): Significant bleeding that won't stop Large, painful swelling Increasing redness spreading from site Signs of infection (heat, pus, severe pain) First injection tip: It's completely normal to feel nervous

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doi: 10.1016/j.ifset.2015.06.008