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Therefore, we compared the genes whose expression level variation was confirmed by RT-qPCR using samples in which opacity was reduced by the addition of HAT inhibitors and Atm inhibitors with those whose expression level variation was confirmed in this study

Clinical research has found that older adults with T2DM are frequently associated with cardio-renal challenges and are more likely to have the risk of hypoglycemia in their frail body as well as merge multiple complications and comorbidities which seriously affect the quality of life and lifespan of the elderly ( FIGURE 1 2.1 The aging process and declining pancreatic function in elderly individuals As the metabolic capacity of the elderly declines, multiple metabolic disturbances combined with vascular aging contribute to the increasing prevalence of vascular complications in diabetes year by year

UVvis spectra were acquired using a Multiskan SkyHigh microplate spectrophotometer (ThermoFisher Scientific) and 96-well plates (Corning) or quartz cuvettes using SkanIt software

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