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Differences in odd- and longer-chain saturated fatty acids were also observed including pentadecanoate (15:0), stearic acid (18:0), and arachidic acid (20:0), which where elevated in the pasture-finished bison, whereas palmitate (16:0) and oleic acid (18:0) where elevated in the pen-finished bison

At 2 h, 4 h, and 24 h following MTX administration, rats were anesthetized using ether, blood samples were collected from the retro-orbital plexus using a sterile hematocrit capillary, and serum was separated for monitoring MTX levels