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Cell culture and bioluminescence analysis T cells, isolated from mice, were activated by CD3/CD28 Streptamer kit (#6-8920, iba, Goettingen, Germany) for 30 min and cultured in RPMI 1640 medium (#8123340, Gibco) containing 10% FBS (#A5669701,Gibco), 1% penicillin/streptomycin, 50 M -mercaptoethanol (Beyotime, Shanghai, China), 1 mM sodium pyruvate (Beyotime) and 100 U/mL interleukin-2 (#CK24, Novoprotein, Suzhou, Jiangsu, China)

Design, synthesis, and biological activities of four angiotensin II receptor ligands with -turn mimetics replacing amino acid residues 35

Finally, in the Morris water maze, although acquisition was initially impaired, differences were not maintained and no effect was observed in the probe test for memory, suggesting that BDNF in this subregion does not have a critical function in spatial memory