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doi: 10.1016/j.expneurol.2008.12.002

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Each compound is listed with its proportion of the total vial content so you know exactly what you're working with before the calculation runs

The pellet was then washed, subjected to lysis buffer for 5 minutes and centrifuged again at 3000 g for 5 min to collect the ER in the soluble fraction, whereas the pellet represented the nucleus fraction