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Importantly, the observed oxidative damage and mitochondrial impairment following GCLC knockdown were reproduced across multiple assays and time points, reinforcing its critical role in redox regulation and cellular viability [31,32,33]

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Statistical analysis A combination of multivariate (orthogonal partial least squares discriminant analysis) and univariate (analysis of variance, linear modeling) approaches was applied to perform group comparisons, following the workflow described by Blaise et al

These phenomena are largely impacted by post-donation hemoglobin- oxygen saturation, which has been noted to vary widely across healthy blood donors