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According to pharmacokinetic data, exogenous exosomes have a short residence time in the human circulation and are susceptible to clearance by macrophages associated with organs of the mononuclear phagocytic system (liver, spleen, and lung) ( The effective dose of exosomes in animal research (about 10 9 10 10 exosomes per kilogram of body weight) presents challenges for direct scaling to humans ( In conclusion, while hyperbaric oxygen combined with exosomes demonstrates enhanced efficacy in facilitating tissue repair in animal models, the application of this combination in human clinical trials encounters several constraints, including exosomes delivery methods, dosage scaling, interspecies variations, and timing of intervention

Despite such complexities, integrative functional approaches are beginning to bridge the gap between genetic association and mechanism

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