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However, Hu et al utilized an rxYFP variant that was not fused to a glutaredoxin and therefore relied on endogenous glutaredoxins for the rapid and complete equilibration with the surrounding glutathione pool

A single exposure of doxorubicin between 0 and 50 M, yields minimal cytosolic GSH depletion (2.5% maximum decrease), which recovers within the prescribed time-span (Fig