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Protein precipitation was performed by adding 10 volumes of 25% MeOH in acetonitrile (ACN), except for the brain homogenate which was extracted in 100% MeOH

Some promising approaches include the following: - Garlic-derived allicin: reduces TMAO in murine models (C57BL/6 mice), suggesting anti-atherosclerotic potential ( - TMA lyase inhibitors: synthetic compounds (e.g., fluoromethylcholine, FMC) that irreversibly inhibit CutC/D ( - Probiotics: specific strains can lower TMAO levels ( 1.5 Experimental approach Here, we report the results of an alternative approach based on the use of postbioticspreparations of inanimate microorganisms and/or their components, which confer health benefits to the hostas inhibitors of intestinal TMA lyase ( Lactobacillus paracasei LMG-S-26420 ferment for 72 h under anaerobic conditions in the presence of a freeze-dried botanical hydroalcoholic extract of garlic ( Allium sativum )