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Standard maintenance therapy for pernicious anaemia involves injections every 2 to 3 months following an initial loading phase of six injections over two weeks

The needle is introduced at an angle perpendicular to the spherical skin plane ( Figure 8A and B )

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The rats were maintained in an animal room with an air-conditioned barrier system at an ambient temperature of 25C 2C, relative humidity of 50% 10%, and a 12 h light/dark cycle