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Qualitative and quantitative determination of ten major saponins in Platycodi Radix by high performance liquid chromatography with evaporative light scattering detection and mass spectrometry

Ultimately, these efforts aim to alleviate hepatic steatosis and prevent the progression of fibrosis

Detection of Antioxidant Enzyme Activities Ascorbate peroxidase activity was detected as described in Dehydroascorbate reductase (DHAR) activity was determined as described in Glutathione reductase activity was detected as described in Preparation of Nonprotein Thiol Extracts The alga (0.2 g of DT) was frozen in liquid nitrogen and homogenized in a mortar with a pestle and extracted by addition of 1.2 ml of 0.1% (w/v) trifluoroacetic acid (TFA)6.3 mM diethylenetriaminepentaacetic acid (DTPA)