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Although not shown directly, increasing macrophage GR expression in vivo may therefore protect macrophage mitochondria from thiol oxidative stress-induced injury, thereby preventing macrophage dysfunction and/or cell death and thus the progression of atherosclerotic lesions

erinaceus up-regulated gene expressions of heme oxygenase-1 (HO-1), -glutamylcysteine synthetase (-GCLC), and glutathione levels, thus contributing to inhibition of reactive oxygen species (ROS) (46)

Allow vials to reach room temperature for 15 to 30 minutes before opening to prevent condensation Disinfect rubber stoppers with alcohol swab and allow complete evaporation Draw the calculated volume of bacteriostatic water into syringe Angle needle against vial wall and slowly inject water down the side, allowing it to gently slide onto powder Never squirt water directly onto lyophilized powder as this damages peptide structure Gently swirl or roll vial between palms to dissolve

Statistical analysis of the data was performed using a Students t -test