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2019;6 doi: 10.1177/2333794X19830696

Additionally, screening small molecule drugs for each reactive cysteine residue site remains a time-consuming and labor-intensive endeavor

Brain tissues were analyzed using H&E staining, RNA sequencing (RNA-seq), Western blotting for key pathway proteins, immunofluorescence for glial cell activation, and ELISA for inflammatory cytokines

Recent methodological advancesincluding organotypic slice cultures, three-dimensional brain organoids and in vivo imaging approachesoffer scalable and physiologically relevant platforms that more faithfully capture astrocyte heterogeneity and functional consequences