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Spermatozoa were collected from the cauda epididymis and incubated in M2 medium for 60 min at 37 C

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For instance, acetylation, O-GlcNAcylation, and ubiquitination have been shown to be involved in insulin gene transcription, and phosphorylation has a role in mediating the signaling cascades needed for insulin secretion (83)

This enzyme is represented by a superfamily of isoenzymes catalyzing the conjugation of glutathione with a wide range of nonpolar compounds of exogenous and endogenous origin containing electrophilic atoms of carbon, sulfur, nitrogen, and phosphorous, which assists in the defense of the cell against possible toxic action of these compounds [84-87]