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These results align with findings from previous flow cytometric studies, particularly the pronounced decline of naive CD8 T cells with age 34,35

While fluorodeoxyglucose positron emission tomography (FDG-PET) remains the gold standard for clinical assessment of cerebral glucose metabolism 2,3 , its focus on glucose uptake provides limited insight into downstream metabolic pathways that directly support neuronal signalling, including the glutamate-GABA cycle that underlies excitatory-inhibitory balance