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Molecular dynamics (MD) simulations of the bacterial heme transporter CydDC have shown that membrane-embedded heme undergoes a ~ 90 rotation at lateral gate during entry into the transporter 52

Subsequently, cells were cultured for the indicated time in RPMI 1640 containing 10% FBS, 100 U/ml penicillin-streptomycin (15140122, Thermo Fisher Scientific) and 50 ng/ml recombinant mouse M-CSF (R&D Systems, Minneapolis, MN, USA) in 6-well plates [80,000 cells/cm ( 2 at 37C