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Cells were collected in PBS and sequentially lysed in buffer 1 (50 mM HEPES pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% TritonX-100), buffer 2 (10 mM Tris pH 8, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA) and buffer 3 (10 mM Tris pH 8, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine) with protease inhibitors (Roche)

FETPY: A diiron(I) thio-carbyne complex with prominent anticancer activity in vitro and in vivo

915,916 Investigations have also demonstrated that muscle atrophy caused by iron overload is connected to the ROS-mediated activation of the ubiquitin-proteasome system

RNA sequencing of microglia and monocyte-derived macrophages from mice with experimental autoimmune encephalomyelitis illustrates a changing phenotype with disease course