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Human TrxR1 and GR share a high active site residue similarity at the cofactor and substrate binding sites as well as a similar reaction mechanism with respect to the reductive half-reaction 10

A portion of each muscle sample was immediately placed in ice-cold buffer X (50 mM K-MES, 7.23 mM K 2 EGTA, 2.77 mM CaK 2 EGTA, 20 mM imidazole, 20 mM taurine, 5.7 mM ATP, 14.3 mM phosphocreatine, and 6.56 mM MgCl 2 -6H 2 O [pH 7.1]) for preparation of permeabilized fiber bundles as previously described (89)