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Not to be confused with iron deficiency anaemia, where there aren't enough healthy red blood cells, pernicious anaemia is an autoimmune disease that prevents your body from absorbing B12 and is the most common cause of vitamin B12 deficiency in the UK

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For quantitative real-time PCR (qPCR), the qPCR mixture, comprising cDNA, SYBR Green master mix (Takara Bio, Dalian, China), and primers, was prepared and amplified using a QuantStudio 7 Flex Real-Time PCR System (Life Technologies, USA)