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Kanamycin-resistant clones were isolated, analysed by PCR for the presence of the dcas9 cassette and tested for the efficiency of knockdown by monitoring the growth of tested strains on 7H10 agar in the presence of the ATc inducer

For example, a previous study found that the levels of miR-221, miR-23a and miR-574 in blood-derived EVs from 41 patients with COPD and 29 healthy individuals were negatively correlated with the FEV1/FVC ratio, with 95% confidence intervals of 0.776, 0.688 and 0.842, respectively (64)