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and resuspended in 20 mM HEPES pH 7.5, 100 mM NaCl, 10 mM MgCl 2 , 5 mM CaCl 2 , 2 mM KCl, 0.1 mM TCEP, supplemented with Protease Inhibitor Cocktail (TargetMol, 1 mL/ 100 mL suspension)

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