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Zhongguo Zhong Xi Yi Jie He Za Zhi (2016) 36(5):55963

The membrane was blocked with 2% BSA overnight and was probed with antibodies against goat anti-FLAG (1:1000, Cell Signalling Technologies, D6W5B, #14793), alpha Tubulin (1:10,000, mAb, mouse) or rabbit anti-HA (1:1000, Cell Signalling Technologies) followed by a compatible HRP-linked secondary antibody

HIF-1 hydroxilated is associated to E3 ubiquitin ligase Von Hippel Lindau protein (VHLp) for its degradation, whereas in hypoxia condition stabilization and nuclear translocation occur, leading to oncogenes activation (192)