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Briefly, the highly denaturing guanidine isothiocyanatecontaining buffer was added to the samples and worms were sonicated in ice performing 2 cycles of 10 seconds at 30% intensity and 1 cycle of 10 seconds at 40% intensity, with 1 minute pause between each cycle

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These nanoparticles have demonstrated the ability to deliver mRNA molecules to hepatocytes with high efficiency, offering potential applications for genetic diseases such as methylmalonic acidemia (MMA) [124], primary hyperoxaluria type 1 (PH1) [125], glycogen storage disease 1a (GSD1a) [126], citrin deficiency [127], acute intermittent porphyria (AIP) [128], maple syrup urine disease [129], arginase deficiency [130], OTCD [131], and progressive familial intrahepatic cholestasis type 3 (PFIC3) [132]