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S-RBD, N, S1, S1-NTD, and S2-ECD conjugated beads were then used as capture beads in flow immunoassay where they were incubated with anti-S-RBD human IgG positive control (provided in GenScript SARS-CoV-2 Spike S1-RBD IgG & IgM ELISA Detection Kit as positive control), recombinant Human ACE2-Fc (Acro Biosystems) or plasma and serum samples for 1 h at room temperature

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