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Each sample was drawn into tubes containing 4 ml of activating gel and allowed to clot at room temperature

M., Caulin, A

A second aminoacid residue of mechanistic importance is Arg15, which is widely conserved among the known alpha class GSTs ( a value of the thiol group, and mutation of Arg15 to Ala or His decreases the steroid isomerase activity by two orders of magnitude, twice the effect of the Tyr9Phe mutation ( The mechanistic studies of human GST A1-1 were extended to human GST A3-3 following the discovery of the latter enzyme ( a value of 6.1 and Tyr9 phenol pK a of 7.9, somewhat lower than the corresponding pK a values of GST A1-1 ( cat value

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