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FIGURE 1 To test the genetic interaction between YAK1 and CYS4 , the coding sequence of YAK1 was amplified from the genomic DNA of a W303 wild-type strain and subcloned into either a pRS416- GPD (centromeric plasmid) or a pRS426- GPD (2 ) vector using primers listed in Supplementary Table 2

Recent studies have pushed forward the frontiers of our knowledge on eryptosis, revealing that mature erythrocytes have a more complex cell death signaling network than conventionally thought

However, homocystinuria may be more frequent in specific populations (1:1800 in Qatar) and molecular epidemiological studies suggest a higher frequency of the adult vitamin B 6 -responsive form [36, 37, 39,40,41,42]

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