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Figure 1 In addition to the ARC, peripheral ghrelin binds to GHSR expressing cells in brain stem regions that regulate feeding in response to rapid changes in metabolic signals like glucose and fatty acids in blood, and that integrate hormonal and neural signals coming from the gut via the ascending branch of the vagus nerve (29)

JC-1 assay 4T1 cells were incubated in confocal culture plates (5 10 4 cells/plate) for 24 h, followed by incubation with different formulations for 24 h

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Glycosylation can lead to protein cross-linking, structural changes and loss of function, which can affect the normal physiological function of cells (53,54)