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Effects of the MTHFR Mutation: Reduced Folate Metabolism: Folate (vitamin B9) is essential for many processes in the body, and MTHFR mutations can hinder the conversion of folate to its active form (5-MTHF)

In addition, a very fresh fecal smear (ideally sampled directly from the rectum) can be placed onto a slide with a drop of saline and looked at under the microscope to observe for movement (giardia may also be seen with this technique and look similar to tritrichomonas, however, tritrichomonas tend to wiggle forward, whereas giardia move in a swirling or 'falling-leaf' pattern)

The use of PPIs is also thought to contribute to vitamin B12 deficiency (Aroda et al, 2016)

When liver function is compromised due to excessive alcohol consumption, it can result in decreased levels of circulating iron and ultimately contribute to anemia