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In order to determine whether this enhanced ROS production was coming from the mitochondria, BT16 cells were treated with cisplatin and stained with Mitosox, a fluorogenic dye specifically targeted to the mitochondria that emits red fluorescence upon oxidation

DIA LCMS analysis For each sample, peptides (250 ng) were injected into an HPLC (nanoElute, Bruker Daltonics, Billerica, MA, USA) and loaded onto a trap column with a constant flow of 4 L/min (Acclaim PepMap100 C18 column, 0.3 mm id 5 mm, Dionex Corporation, Sunnyvale, CA, USA) then eluted onto an analytical C18 Column (1.9 m beads size, 75 m 25 cm, PepSep, city) heated at 50 C

Whichever protocol is chosen, tretinoin should always be paired with diligent daily broad-spectrum sunscreen , since retinoids increase photosensitivity

Imaging findings showed that 83% of stage I patients and 48% of stage II patients had controlled tumors