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Cells were incubated with a mouse monoclonal antibody targeting the human transferrin receptor (Clone H68.4, #136800, Invitrogen, Eugene, OR) diluted to a final concentration of 2 g/mL in PBS overnight at 4 C

Although ferroptosis is often described as an iron-dependent cell death pathway, an explicit increase in the labile iron pool is not universally required as a trigger

& Hamilton, J

In AD, chronically overactivated microglia release proinflammatory cytokines, such as IL-1 and TNF-, generating a self-perpetuating cycle of inflammation and neuronal injury