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Analytical Techniques for Methionine Cycle Study Mass Spectrometry (MS): A powerful tool for quantifying key metabolites such as SAM, SAH, homocysteine, and 5-methyltetrahydrofolate (5-MTHF), providing insights into metabolic balance

Baseline oxidative stress and glutathione biomarkers should be measured before starting supplementation, then rechecked every 812 weeks

Our study found that severely elevated OxS (as a likely consequence of GSH deficiency) in the heart, liver and kidneys of old mice improved only with GlyNAC supplementation, and not with isocaloric-isonitrogenous placebo diets

Moreover, the prevailing reliance on animal models in the extant literature, coupled with the insufficient validation of clinical trial findings, accentuates the critical need for bridging the translational divide between preclinical insights and clinical application