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Turk J Biol 36:8592 Pillco A (2011) Evaluacin toxicolgica de mezclas complejas mediante ensayos alternativos a la experimentacin animal de genotoxicidad, mutagenicidad y ecotoxicidad (Toxicological evaluation of complex mixtures employing genotoxicity, mutagenicity and ecotoxicity assays as an alternative to animal testing) Doctoral thesis, Autonomous University of Madrid, p 173 Rank J, Nielsen MH (1998) Genotoxicity testing of wastewater sludge using the Allium cepa anaphase-telophase chromosome aberration assay

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4 Discussion Selenium, iron, and vitamin E intake were increased in supplemented animals throughout the experimental period and changes in the concentrations of blood metabolites of animals were observed, indicating their supportive role against pathogens

Apart from collagen degradation owing to excessive MMP expression, ROS also triggers a decrease in collagen production by inhibiting the TGF- signal pathway (75)