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STING induces LC3B lipidation onto single-membrane vesicles via the V-ATPase and ATG16L1-WD40 domain

Methods: Our study utilized C57BL/6 mice models for hepatic fibrosis induced by both CCl4 administration and a high-fat diet, mimicking metabolic dysfunction-associated steatohepatitis (MASH)

Because the bound copper is integral to the molecule's identity, the mass measured by LC-MS reflects the intact complex, confirming that the copper coordination has been retained through synthesis and purification