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doi: 10.1038/cr.2015.68 57 Palsson-McDermottEMCurtisAMGoelGLauterbachMASheedyFJGleesonLEet al

Effects of arginine replacement with L-citrulline on the arginine/nitric oxide metabolism in chickens: an animal model without urea cycle

After affinity selection of the fusion protein on the chitin matrix, the intein undergoes specific cleavage by thiol reagents or changes in pH and temperature, thereby releasing the target protein from the chitin-bound tag