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In terms of the recovery, purity and functional integrity of the bacteria, Nycodenz has been shown to be superior both to inorganic salts such as CsCl and Na2WO4 and other non-ionic gradient media such as metrizamide and Percoll [2,3]

Cold water also could contain more dissolved gases, which lower its freezing point

We used a simple method based on the incorporation of PI for red fluorescence staining that does not require an extensive knowledge of schistosome biology [40]