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However, this random conjugation strategy leads to a heterogeneous distribution of drug-to-antibody ratios (DARs), typically ranging from 0 to 8, and the uncertainty of conjugation sites may affect antigen binding or pharmacokinetic stability

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However, it requires highly specific antibodies, still involves relatively high sequencing costs, and is less effective at distinguishing specific methylation regions compared to other methods (66).In a multicenter study conducted in Italy, MeDIP-seq was used to analyze 26 affected and 26 unaffected relatives from 8 MS families (67)

Lancet Neurol 23(11):11191132