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Statistical analyses were performed using either an un-paired t -test assuming equal variance, one-, or two-way ANOVA, as appropriate, and are specifically indicated on each figure legend

Beginning in July 2014, after giving his informed consent, the patient underwent the following treatment: Combined therapy with oral antioxidants propolis 600 mg + bilberry 160 mg + silymarin 400 mg + ginkgo biloba 250 mg + L-carnitine 1000 mg + coenzyme Q10 100 mg + Boswellia 200 mg + vitamin E 30 mg/daily/ + opical diclofenac gel 4%/2 daily for 12 months