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the body's sensitivity to insulin is reduced, so that the biological effect of insulin on target organs is reduced and glucose uptake and utilization are reduced, thus failing to maintain blood glucose stability

The mechanism: IGF-1 receptor activation in renal tubules increases sodium reabsorption, expanding plasma volume and driving fluid into interstitial spaces

The plate was then covered with plastic film and incubated for 1 h at 37 C in a shaker incubator

Absorbance was measured using a spectrophotometer according to the instructions provided in each kit, and the enzyme activities were calculated based on the corresponding standard operating procedures to ensure reproducibility