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To reconstitute its blue-colored copper complex, the Azurin concentration in the eluate was determined from its absorbance at 280 nm, and 100 mM CuSO 4 was slowly added at a tenfold molar ratio

They may result from: 1) an inhibition of the homocysteine remethylation pathway that is a consequence of folate or vitamin B 12 deficiencies, or the presence of the thermolabile MTHFR mutation or a functional deficiency in methionine synthase

Subcutaneous may offer slightly more consistent plasma levels, but the practical difference is marginal given the compounds short half-life requiring daily dosing either way