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Finally, we performed transmission electron microscopy (TEM) to detect autophagic vesicles at the ultrastructural level

After washing with PBS, 10 M DCF-DA was loaded into the wells and incubated at 37 C for an additional 20 min

Key among these is the acute transcriptional activation of genes harboring CNC-sMaf binding elements (CsMBE), many of which encode cytoprotective proteins that neutralize free radicals and limit cellular damage