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10.1073/pnas.0605296103 Proc

In the present study, we investigated whether the Nrf2/GSH pathway is a critical determinant in cytotoxicity mediated by cisplatin in MRT

After centrifugation at 18,000 rpm for 30 min, the supernatant was collected and incubated with glutathione-Sepharose 4B at 4 C, followed by washing with PBS buffer