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Additionally, existing ferroptosis inducers suffer from off-target toxicity and poor tumor penetration, which are further aggravated by the physical barriers and metabolic properties of the TME ( Future research should integrate ferroptosis with TME remodeling and immunotherapy

Investigators designing cancer biology studies should carefully consider the mechanistic profile of each compound before selection

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Antibodies from Abcam: NLRP3 (ab263899), Apoptosis associated speck-like protein (ASC, ab175449), GSDMD (ab209845), IL-18 (ab191860), IL-1 (ab254360), Caspase-1 (ab286125), Phosphatidylinositol 3-kinase (PI3K, ab1916061), Phosphorylation PI3K (p-PI3K, ab182651), Protein kinase B (AKT, ab8805), Phosphorylation AKT (p-AKT, ab38449), NF-B (ab16502), Phosphorylation NF-B (p-NF-B, Catalog No: ab76302), NF-B inhibitory protein (IB-, ab32518), Glial fibrillary acidic protein (GFAP, ab7260), Ionized calcium-binding adapter molecule 1 (IBA1, ab178847), Antibodies from Wuhan Sanying Biotechnology Co., Ltd.: Cleaved-Caspase-1 (AF4022), -actin internal reference (20536-1-AP), GAPDH internal reference (60004-1-lg)