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Cell culture was performed under aseptic conditions in Class II biosafety cabinets and incubation took place at 37 o C and 5% CO 2 in a humidified incubator

Tai Y, Zhang Z, Liu Z, Li X, Yang Z, Wang Z, et al

It is commonly used in experimental models investigating neurotransmitter regulation, appetite-related signaling pathways, and neurochemical mechanisms under controlled research conditions