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Finally, the lysate was centrifuged at 12,000 g for 30 min, and the supernatant containing the total protein solution was collected for subsequent experiments

The proposed expert opinion planned to provide a practical and implementable guidance document addressing DPN in terms of: a) definition, pathophysiology and risk factors, b) clinical manifestations and diagnosis (current challenges, a proposed screening and diagnostic algorithm), c) screening for early diagnosis and symptom progression, and d) management of disease in terms of the optimal diabetes treatment (intensive glycemic control, lifestyle modification and multifactorial risk intervention), the pathogenetically oriented pharmacotherapy (with special emphasis on alpha lipoic acid), and the symptomatic pain relief (in painful DPN)

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Deferasirox-dependent iron chelation enhances mitochondrial dysfunction and restores p53 signaling by stabilization of p53 family members in leukemic cells