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For each assay, cells were plated in duplicate, with one plate containing CellTox Green Dye added according to the Express, No-Step Addition at Seeding protocol described in the CellTox Green Cytotoxicity Assay Technical Manual #TM375 (10l of CellTox Green Dye per 5ml of cells)

Research has demonstrated that GA modulates the AMPK/SREBP/ACC and IRs-1/Akt signaling pathways, which are pivotal in cellular metabolism, and it also suppresses liver inflammation by targeting the IKK/i-B/NF-B signaling pathway, a key mediator of inflammatory responses ( Previous studies on the mechanism of action of GA on NAFLD were based on pharmacological methods

Weekly injections are common during intense training periods, followed by bi-weekly or monthly doses for recovery and maintenance