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Where TGF-1 activates fibroblasts through Smad2/3 signaling transduction, which results in increased expression of ECM components (fibronectin and vimentin) that further participate in the proliferation, migration, and trans-differentiation of fibroblasts into myofibroblasts that express a high level of -SMA 65 , VCN managed to reduce the expression of the fibrotic indices for ECM deposition and the signaling protein (Smad2/3) involved in the activation of fibroblasts and transitioning into ECM-producing myofibroblasts in MRC-5 induced with TGF-1

doi: 10.1016/j.jad.2016.12.048, 51