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This approach allowed the simultaneous introduction of 3 shRNA targeting FADS1 and 3 shRNA targeting FADS2 in 4T1 cells and the generation of stable FADS1/FADS2 double knockdown transfected cells (FADS1/2 KD 4T1, Fig

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Polyunsaturated fatty acids can be further grouped into clusters based on the position of their first double bond, delineated as n-3, n-6, n-7, and n-9